Identification of the glycosylation sites of Opsin3 and its glycosylation modification function / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1173-1182, 2022.
Article
in Chinese
| WPRIM
| ID: wpr-927772
ABSTRACT
Opsin3 (OPN3) is a photoreceptor membrane protein with a typical seven-alpha helical transmembrane structure that belongs to the G-protein-coupled receptor (GPCR) superfamily and is widely expressed in brain. In recent years, it has been reported that OPN3 is also highly expressed in adipose tissue, and the protein is associated with the production of skin melanin. We found that the N82 site is the glycosylation site of OPN3. SNAP-tagTM has diverse functions and can be applied to a variety of different studies. By constructing a SNAP-tagged OPN3 recombinant protein, the distribution position of SNAP-OPN3 in cells can be clearly observed by fluorescence confocal microscopy using SNAP-Surface® 549 and SNAP-Cell® OregonGreen®, which provides a new method for studying the function of OPN3. It also shows that SNAP-tag does not affect the function of OPN3. Using the SNAP tag we found that OPN3 cannot be taken up to the cell membrane after glycosylation site mutation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Glycosylation
/
Cell Membrane
/
Melanins
/
Membrane Proteins
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2022
Type:
Article
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