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Protective effect of ginsenoside Rg_1 aganist diabetic retinopathy by inhibiting NLRP3 inflammasome in type 2 diabetic mice / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 476-483, 2022.
Article in Chinese | WPRIM | ID: wpr-927992
ABSTRACT
Ginsenoside Rg_1, one of the main active components of precious traditional Chinese medicine Ginseng Radix et Rhizoma, has the anti-oxidative stress, anti-inflammation, anti-aging, neuroprotection, and other pharmacological effects. Diabetic retinopathy(DR), the most common complication of diabetes, is also the main cause of impaired vision and blindness in the middle-aged and the elderly. The latest research shows that ginsenoside Rg_1 can protect patients against DR, but the protection and the mechanism are rarely studied. This study mainly explored the protective effect of ginsenoside Rg_1 against DR in type 2 diabetic mice and the mechanism. High fat diet(HFD) and streptozotocin(STZ) were used to induce type 2 diabetes in mice, and hematoxylin-eosin(HE) staining was employed to observe pathological changes in the retina of mice. The immunohistochemistry was applied to study the localization and expression of nucleotide-binding oligomerization domain-like receptors 3(NLRP3) and vascular endothelial growth factor(VEGF) in retina, and Western blot was used to detect the expression of nuclear factor-kappa B(NF-κB), p-NF-κB, NLRP3, caspase-1, interleukin-1β(IL-1β), transient receptor potential channel protein 6(TRPC6), nuclear factor of activated T-cell 2(NFAT2), and VEGF in retina. The results showed that ginsenoside Rg_1 significantly alleviated the pathological injury of retina in type 2 diabetic mice. Immunohistochemistry results demonstrated that ginsenoside Rg_1 significantly decreased the expression of NLRP3 and VEGF in retinal ganglion cells, middle plexiform layer, and outer plexiform layer in type 2 diabetic mice. According to the Western blot results, ginsenoside Rg_1 significantly lowered the expression of p-NF-κB, NLRP3, caspase-1, IL-1β, TRPC6, NFAT2, and VEGF in retina of type 2 diabetic mice. These findings suggest that ginsenoside Rg_1 can significantly alleviate DR in type 2 diabetic mice, which may be related to inhibition of NLRP3 inflammasome and VEGF. This study provides experimental evidence for the clinical application of ginsenoside Rg_1 in the treatment of DR.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Signal Transduction / NF-kappa B / Ginsenosides / Vascular Endothelial Growth Factor A / Diabetes Mellitus, Experimental / Diabetes Mellitus, Type 2 / Diabetic Retinopathy / Inflammasomes / NLR Family, Pyrin Domain-Containing 3 Protein Limits: Animals / Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2022 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Signal Transduction / NF-kappa B / Ginsenosides / Vascular Endothelial Growth Factor A / Diabetes Mellitus, Experimental / Diabetes Mellitus, Type 2 / Diabetic Retinopathy / Inflammasomes / NLR Family, Pyrin Domain-Containing 3 Protein Limits: Animals / Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2022 Type: Article