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Value of metagenomic next-generation sequencing in children with severe infectious diseases / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics ; (12): 273-278, 2022.
Article in English | WPRIM | ID: wpr-928599
ABSTRACT
OBJECTIVES@#To study the application value of metagenomic next-generation sequencing (mNGS) in children with severe infectious diseases.@*METHODS@#An analysis was performed on the clinical data and laboratory test results of 29 children with severe infection who were admitted to the Second Affiliated Hospital of Wenzhou Medical University from June 2018 to December 2020. Conventional pathogen culture was performed for the 29 specimens (27 peripheral blood specimens and 2 pleural effusion specimens) from the 29 children, and mNGS pathogen detection was performed at the same time.@*RESULTS@#Among the 29 children, 2 tested positive by conventional pathogen culture with 2 strains of pathogen, and the detection rate was 7% (2/29); however, 20 children tested positive by mNGS with 38 strains of pathogen, and the detection rate was 69% (20/29). The pathogen detection rate of mNGS was significantly higher than that of conventional pathogen culture (P<0.05), and mNGS could detect the viruses, fungi, and other special pathogens that conventional pathogen culture failed to detect, such as Orientia tsutsugamushi. The univariate analysis showed that gender, routine blood test results, C-reactive protein, procalcitonin, D-dimer, radiological findings, and whether antibiotics were used before admission did not affect the results of mNGS (P>0.05).@*CONCLUSIONS@#Compared with conventional pathogen culture, mNGS is more sensitive for pathogen detection, with fewer interference factors. Therefore, it is a better pathogenic diagnosis method for severe infectious diseases in children.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Communicable Diseases / Sensitivity and Specificity / Metagenomics / High-Throughput Nucleotide Sequencing / Anti-Bacterial Agents Type of study: Diagnostic study Limits: Child / Humans Language: English Journal: Chinese Journal of Contemporary Pediatrics Year: 2022 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Communicable Diseases / Sensitivity and Specificity / Metagenomics / High-Throughput Nucleotide Sequencing / Anti-Bacterial Agents Type of study: Diagnostic study Limits: Child / Humans Language: English Journal: Chinese Journal of Contemporary Pediatrics Year: 2022 Type: Article