The signal sequence of type II porcine reproductive and respiratory syndrome virus glycoprotein 3 is sufficient for endoplasmic reticulum retention
Journal of Veterinary Science
;
: 307-313, 2013.
Article
in English
| WPRIM
| ID: wpr-92900
ABSTRACT
The glycoprotein 3 (GP3) of type II porcine reproductive and respiratory syndrome virus has the characteristic domains of a membrane protein. However, this protein has been reported to be retained in the endoplasmic reticulum (ER) rather than transported to the plasma membrane of the cell. In this study, we performed confocal laser scanning microscopy analysis of variants of GP3 and foundthat the signal sequence of the GP3 led to confinement of GP3 in the ER, while the functional ortransmembrane domain did not affect its localization. Based on these results, we concludedthat the signal sequence of GP3 contains the ER retention signal, which might play an important role in assembly of viral proteins.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Protein Sorting Signals
/
Cell Line
/
Cell Membrane
/
Viral Envelope Proteins
/
Microscopy, Confocal
/
Porcine respiratory and reproductive syndrome virus
/
Sequence Analysis, Protein
/
Endoplasmic Reticulum
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2013
Type:
Article
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