Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 84-88, 2022.
Article
in English
| WPRIM
| ID: wpr-929041
ABSTRACT
This study aimed to characterize the chemical composition of a new sulfated polysaccharide from the red alga Gracilaria chouae and evaluate its activation effects on RAW264.7 macrophages. It showed that the obtained G. chouae polysaccharide (GCP-3A) was a sulfated acidic polysaccharide with a molecular weight of 11.87 kDa. GCP-3A was composed of xylose, galactose, glucose, and mannose with a molar ratio of 3.0029.280.630.45, and it contained α,β-glycosidic linkages. Scanning electron microscopy (SEM) and a Congo red test showed that it was a heterogeneous polysaccharide with irregular interwoven sheets and rods, and did not have a triple-helix conformation. Furthermore, GCP-3A significantly promoted the proliferation of RAW264.7 macrophages and the secretion of nitric oxide (NO) in tests of 3-(4,5-dimethylthiahiazo-2-yl)-2,5-diphenytetrazoliumromide(MTT) and NO.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polysaccharides
/
Sulfates
/
Gracilaria
/
Macrophages
/
Molecular Weight
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2022
Type:
Article
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