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Mitochondrial and DNA damage in bovine somatic cell nuclear transfer embryos
Journal of Veterinary Science ; : 235-240, 2013.
Article in English | WPRIM | ID: wpr-92909
ABSTRACT
The generation of reactive oxygen species (ROS) and subsequent mitochondrial and DNA damage in bovine somatic cell nuclear transfer (SCNT) embryos were examined. Bovine enucleated oocytes were electrofused with donor cells and then activated by a combination of Ca-ionophore and 6-dimethylaminopurine culture. The H2O2 and .OH radical levels, mitochondrial morphology and membrane potential (DeltaPsi), and DNA fragmentation of SCNT and in vitro fertilized (IVF) embryos at the zygote stage were analyzed. The H2O2 (35.6 +/- 1.1 pixels/embryo) and .OH radical levels (44.6 +/- 1.2 pixels/embryo) of SCNT embryos were significantly higher than those of IVF embryos (19.2 +/- 1.5 and 23.8 +/- 1.8 pixels/embryo, respectively, p < 0.05). The mitochondria morphology of SCNT embryos was diffused within the cytoplasm. The DeltaPsi of SCNT embryos was significantly lower (p < 0.05) than that of IVF embryos (0.95 +/- 0.04 vs. 1.21 +/- 0.06, red/green). Moreover, the comet tail length of SCNT embryos was longer than that of IVF embryos (515.5 +/- 26.4 microm vs. 425.6 +/- 25.0 microm, p < 0.05). These results indicate that mitochondrial and DNA damage increased in bovine SCNT embryos, which may have been induced by increased ROS levels.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: DNA Damage / DNA, Mitochondrial / Fertilization in Vitro / Reactive Oxygen Species / Apoptosis / Colorimetry / Microscopy, Confocal / In Situ Nick-End Labeling / Comet Assay / Embryo, Mammalian Limits: Animals Language: English Journal: Journal of Veterinary Science Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: DNA Damage / DNA, Mitochondrial / Fertilization in Vitro / Reactive Oxygen Species / Apoptosis / Colorimetry / Microscopy, Confocal / In Situ Nick-End Labeling / Comet Assay / Embryo, Mammalian Limits: Animals Language: English Journal: Journal of Veterinary Science Year: 2013 Type: Article