Development and functional verification of CAR-T cells targeting CLL-1 / 中华血液学杂志
Chinese Journal of Hematology
; (12): 102-106, 2022.
Article
in Zh
| WPRIM
| ID: wpr-929540
Responsible library:
WPRO
ABSTRACT
Objective: To explore the development of a CAR-T cells targeting CLL-1 and verify its function. Methods: The expression levels of CLL-1 targets in cell lines and primary cells were detected by flow cytometry. A CLL-1 CAR vector was constructed, and the corresponding lentivirus was prepared. After infection and activation of T cells, CAR-T cells targeting CLL-1 were produced and their function was verified in vitro and in vivo. Results: CLL-1 was expressed in acute myeloid leukemia (AML) cell lines and primary AML cells. The transduction rate of the prepared CAR T cells was 77.82%. In AML cell lines and AML primary cells, CLL-1-targeting CAR-T cells significantly and specifically killed CLL-1-expressing cells. Compared to untransduced T cells, CAR-T cells killed target cells and secreted inflammatory cytokines, such as interleukin-6 and interferon-γ, at significantly higher levels (P<0.001) . In an in vivo human xenograft mouse model of AML, CLL-1 CAR-T cells also exhibited potent antileukemic activity and induced prolonged mouse survival compared with untransduced T cells [not reached vs 22 days (95%CI 19-24 days) , P=0.002]. Conclusion: CAR-T cells targeting CLL-1 have been successfully produced and have excellent functions.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Receptors, Mitogen
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T-Lymphocytes
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Leukemia, Myeloid, Acute
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Cytokines
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Immunotherapy, Adoptive
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Lectins, C-Type
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Cell Line, Tumor
Limits:
Animals
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Humans
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2022
Type:
Article