Establishment of a platelet production model by bone marrow cavity transplantation of mouse primary megakaryocytes / 中华血液学杂志
Chinese Journal of Hematology
; (12): 272-278, 2022.
Article
in Zh
| WPRIM
| ID: wpr-929635
Responsible library:
WPRO
ABSTRACT
Objective: To establish an intramedullary transplantation model of primary megakaryocytes to evaluate the platelet-producing capacity of megakaryocytes and explore the underlying regulatory mechanisms. Methods: Donor megakaryocytes from GFP-transgenic mice bone marrow were enriched by magnetic beads. The platelet-producing model was established by intramedullary injection to recipient mice that underwent half-lethal dose irradiation 1 week in advance. Donor-derived megakaryocytes and platelets were detected by immunofluorescence staining and flow cytometry. Results: The proportion of megakaryocytes in the enriched sample for transplantation was 40 to 50 times higher than that in conventional bone marrow. After intramedullary transplantation, donor-derived megakaryocytes successfully implanted in the medullary cavity of the recipient and produce platelets, which showed similar expression of surface markers and morphology to recipient-derived platelets. Conclusion: We successfully established an in vivo platelet-producing model of primary megakaryocytes using magnetic-bead enrichment and intramedullary injection, which objectively reflects the platelet-producing capacity of megakaryocytes in the bone marrow.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Blood Platelets
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Bone Marrow
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Bone Marrow Cells
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Megakaryocytes
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Bone Marrow Transplantation
Limits:
Animals
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Humans
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2022
Type:
Article