Biocompatibility evaluation of collagen suture and collagen biofilm / 国际生物医学工程杂志

ABSTRACT

Objective:To evaluate the biocompatibility of collagen suture (CS) and collagen biofilm (CB) preliminarily.Methods:The pyrogenic contaminants test was used to analyze the pyrogen in CS and CB. The skin stimulation and intradermal stimulation tests were used to evaluate the stimulation effects of CS and CB to the skin. The hemolytic test was used to evaluate the hemolytic effect of CS and CB. The muscle implantation experiment was used to evaluate the stimulation and toxicity of CS and CB.Results:The results of pyrogenic contaminants test show that the temperature increment of rabbits in each group is lower than 0.6 ℃, and the total temperature increment is lower than 1.4 ℃ indicating that the two materials meet the requirements of pyrogenic examination and the pyrogenic contaminants test is qualified. The results of skin stimulation test and intradermal stimulation test of collagen suture and collagen biofilms were negative indicating that the two materials have no skin irritation. The hemolysis rates of collagen suture and collagen biofilm were 2.943% and 4.127% respectively (all P<0.05) indicating that the two materials will not cause hemolysis. The muscle was tolerated well and the tissue response was not serious after two biomaterials were embedded, which was reduced over time gradually. Conclusions:Both the collagen suture and collagen biofilm have good biocompatibility.