Long non-coding RNA lnc-SLC2A12-10:1 derived from tumor-associated macrophage exosome aggravates the proliferation and invasion of breast cancer cells through miR-296-5p / 中华内分泌外科杂志

ABSTRACT

Objective:To investigate the effect of the mechanism of lnc-SLC2A12-101 derived from tumor-associated macrophages (TAMs) exosomes on the proliferation and invasion of breast cancer (BC) cells.Methods:GEO microarray analysis was used to screen out the differentially expressed lnc-SLC2A12-101 in BC. qRT-PCR was performed to determine the expression level of lnc-SLC2A12-101 and miR-296-5p in tissue and cells. Then TAMs and exosomes were isolated. After, interfering the expression level of lnc-SLC2A12-101 in exosomes and miR-296-5p expression in cells, then cell proliferation and invasion were detected with the help of MTT and Transwell assays.Results:Compared with adjacent tissues, lnc-SLC2A12-101 was significantly up-regulated in BC tissues ( t=7.09, P<0.001) . Compared with normal breast cells, the expression of lnc-SLC2A12-101 in T47D and MDA-MB-468 cells was significantly up-regulated ( t=9.90, P<0.001) ( t=12.18, P<0.001) . lnc-SLC2A12-101 could act as a ceRNA of miR-296-5p. Knockdown of lnc-SLC2A12-101 inhibited BC cell proliferation and invasion, miR-296-5p inhibitor promoted BC cell proliferation and invasion, but this effect could be partially rescued by si--lnc-SLC2A12-101-Exo (all P<0.05) . Conclusion:lnc-SLC2A12-101 derived from TAMs exosomes promotes BC cell proliferation, invasion and thus advance BC progression.