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Expression of IGHG1 in pancreatic cancer and its effect on cell biological function / 中华内分泌外科杂志
Chinese Journal of Endocrine Surgery ; (6): 211-215, 2022.
Article in Chinese | WPRIM | ID: wpr-930329
ABSTRACT

Objective:

To investigate the expression of immunoglobulin heavy constant gamma 1 (IGHG1) in pancreatic cancer and its effects on proliferation, migration and invasion of pancreatic cancer cell line PANC-1.

Methods:

From Jun. 2018 to Dec. 2020, 65 patients with pancreatic cancer who underwent surgical treatment at Xiang Yang No.1 People’s Hospital, Affiliated Hospital of Hubei University of Medicine were selected. The tumor tissue and normal adjacent tissues were removed, and the expression level of IGHG1 mRNA in pancreatic cancer tissues and adjacent tissues was detected by real-time quantitative PCR (RT-qPCR) . The correlation between IGHG1 expression and clinicopathological features of pancreatic cancer was analyzed. PANC-1 cells were divided into control group, IGHG1 negative control group (si-NC) and interference IGHG1 expression group (si-IGHG1) . IGHG1 mRNA expression, proliferation, migration, invasion and apoptosis of PANC-1 cells, as well as epithelial-mesenchymal transition (EMT) -related proteins E-cadherin and N-cadherin, Vimentin and Bax, Bcl-2, caspase3 protein expression were detected.

Results:

The expression level of IGHG1 mRNA in pancreatic cancer tissue was 2.47±0.23, significantly higher than 1.03±0.12 in adjacent tissues ( P<0.05) . the expression of IGHG1 was correlated with tumor differentiation, TNM stage and lymph node metastasis ( P<0.05) . Compared with the control group and si-NC group, the apoptosis rate of PANC-1 cells in the si-IGHG1 group was [ (5.34±0.65) %, (5.54±0.81) % vs (45.62±2.84) %]. Bax[0.34 ±0.05, 0.32±0.04 vs 1.13±0.12], caspase3 [0.43±0.05, 0.45±0.06 vs 1.22±0.13], and E-cadherin [0.78±0.12, 0.81±0.11 vs 1.34±0.08] protein expression levels increased significantly ( P<0.05) . The protein expression level was significantly increased ( P<0.05) , the expression level of IGHG1 mRNA [2.67±0.23, 2.61±0.21 vs 0.87±0.11] and protein [0.97±0.11, 1.01±0.10 vs 0.51±0.04], cell survival rate [ (98.21±0.56) %, (97.89±0.67) % vs (46.67±1.23) %], migration [ (76.12±2.72) %, (74.23±3.41) % vs (28.55±2.63) %] and invasion [ (85.32±3.71) %, 83.27±3.45) % vs (37.58±2.63) %] ability, and N-cadherin[1.12±0.13, 1.04±0.11 vs 0.61±0.08%], Vimentin[1.03±0.11, 0.97± 0.09 vs 0.49±0.07], and Bcl-2[0.87±0.08, 0.89±0.09 vs 0.62±0.07] protein expression levels were significantly reduced ( P<0.05) .

Conclusions:

The expression of IGHG1 is increased in pancreatic cancer, which is related to the occurrence and development of pancreatic cancer. Interference with IGHG1 expression can inhibit the proliferation, migration, invasion and EMT of pancreatic cancer cells.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Endocrine Surgery Year: 2022 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Endocrine Surgery Year: 2022 Type: Article