Effects of miR-363-5p on proliferation and apoptosis of nasopharyngeal carcinoma cells and the possible mechanism / 中国基层医药

ABSTRACT

Objective:To investigate the effects of miR-363-5p on the proliferation and apoptosis of nasopharyngeal carcinoma cells and the possible mechanism.Methods:miR-363-5p expression in human normal nasopharyngeal epithelial cells NP-69 and nasopharyngeal carcinoma cells 5-8F was detected using quantitative real-time polymerase chain reaction. Proliferation and apoptosis of 5-8F cells overexpresing miR-365-5p were determined. At the same time, Caspase3 and BRD4 protein expression in 5-8F cells were also detected.Results:miR-365-5p expression in 5-8F cells (0.71 ± 0.45) was significantly lower than that in NP-69 cells ( t = 2.68, P < 0.05). After overexpressing miR-363-5p, the proliferation of 5-8F cells was significantly decreased ( F = 22.68, P < 0.05). The apoptotic rate in the 5-8F cells was significantly higher than that in the control group [(24.45 ± 5.38)% vs. (18.23 ± 2.41)%, t = 4.13, P < 0.05]. Bax and Caspase3 protein levels in the 5-8F cells were (1.35 ± 0.24) and (1.44 ± 0.34) respectively, which were significantly higher than those in the NP-69 cells [(1.00 ± 0.08), (1.00 ± 0.23), t = 3.12, 5.12, P < 0.05]. BRD4 protein level in the 5-8F cells was significantly lower than that in the control group [(0.42 ± 0.24) vs. (1.00 ± 0.37), t = 2.98, P <0.05]. Conclusion:miR-365-5p can inhibit proliferation of nasopharyngeal carcinoma cells and promote their apoptosis. The negative regulatory effects of miR-363-5p on tumor cells are achieved possibly through inhibiting BRD4 protein expression.