Effect of AT-rich interactive domain 1A gene overexpression on glioblastoma cell invasion / 中国医师杂志

ABSTRACT

Objective:To investigate the role and mechanism of AT-rich interactive domain 1A gene (ARID1A) in glioblastoma invasion.Methods:Human glioblastoma cell line U87 was cultured in vitro. U87 cells transfected with recombinant pcDNA3.1 (+ )-ARID1A by lipofectamine liposome method as ARID1A overexpression group, and U87 cells transfected with empty plasmid as vector group. Untreated U87 cells were as blank control group. The transfection efficiency was verified by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) and Western blot. Transwell invasion test was used to detect the invasion ability of cells, and Western blot was used to detect the protein expression of c-myc, matrix metalloproteinases (MMP)-2 and MMP-9. Results:48 hours after transfection with ARID1A eukaryotic expression plasmid, the cell invasion ability of ARID1A overexpression group, vector group and blank control group were (42.2±11.5)%, (98.6±4.8)%, (100.0±5.1)%. There was significant difference between ARID1A overexpression group and the other two groups ( P<0.01); The expressions of c-myc, MMP-2 and MMP-9 in ARID1A overexpression group were lower than those in vector group and blank control group ( P<0.01). Conclusions:ARID1A can inhibit the invasion of glioblastoma by inhibiting the expression of MMP-2/MMP-9, and can be used as a potential therapeutic target for glioblastoma.