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Screening of radiation sensitive lipids in rat plasma at early stage after total body irradiation with 60Co γ-rays / 中华放射医学与防护杂志
Chinese Journal of Radiological Medicine and Protection ; (12): 175-180, 2022.
Article in Chinese | WPRIM | ID: wpr-932581
ABSTRACT

Objective:

To explore the characteristics of lipid metabolism in rat plasma after total body irradiation(TBI) in order to provide scientific evidence of radiation biomarkers.

Methods:

For the non-targeted lipidomics study, 50 SD rats were divided into 6 groups and irradiated with 0, 1, 2, 3, 5 or 8 Gy 60Co γ-rays, respectively. For the targeted lipidomics study, 25 rats were divided into 5 groups and irradiated with 0, 0.5, 2.5, 4 or 6 Gy. Venous blood samples were collected and plasma were separated 4 h after TBI. Radiation-sensitive lipids were screened and their concentrations were determined. Receiver operating characteristic curve (ROC) and dose-response were analyzed.

Results:

A total of 15 radiation differential lipids were screened out based on non-targeted lipidomics study and 7 of them were identified as radiosensitive lipids by targeted lipidomics analysis. The ROC of radiosensitive lipids distinguished area under curve (AUC) of samples in 0 Gy group and > 0 Gy group, < 2 Gy group and ≥ 2 Gy group were all > 0.75. The AUC values were increased to 0.96 and 0.94 after the panel of radiation sensitive lipids ROC analysis. The concentrations of LysoPC(18 2), LysoPC(22 0), PC(18 0/18 2), PE(18 2/16 0) and PE(18 2/18 0) decreased with irradiation dose within 0-6 Gy.

Conclusions:

A total of 7 plasma radiosensitive lipids in rat plasma were identified 4 h after TBI, and the panel of them could be used for specific dose classification. Five of the lipids had good dose-response relationship.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Prognostic study / Screening study Language: Chinese Journal: Chinese Journal of Radiological Medicine and Protection Year: 2022 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Prognostic study / Screening study Language: Chinese Journal: Chinese Journal of Radiological Medicine and Protection Year: 2022 Type: Article