Effects of the b-FGF to Early Revascularization and Epithelial Regeneration in the Rabbit's Tracheal Autograft / 대한흉부외과학회지

ABSTRACT

Donor airway ischemia is a significant problem after tracheal replacement with homograft or lung transplantation. Several factors such as omentopexy, heparin, PGI2 and fibroblast growth factor, have been shown to induce angiogenesis in vitro and in vivo. This study was designed to investigate whether omentopexy and basic fibroblast growth factor can enhance rabbit tracheal revascularization and epithelial regeneration. Three different experiments were performed with New Zealand white rabbit. In group I(n=15 control group), only cervical tracheal autotransplantation was done. In group II(n=15), cervical tracheal autotransplantation with omentopexy was done through subcutaneous route. In group III(n=15), cervical tracheal autotransplantation was done and 1ug basic fibroblast growth factor was applied. After 3, 7 and 14 days, the animals were sacrificed. The extent of revascularization was investigated by means of uptake of the human serum albumin labelled with 99m technetium, and epithelial regeneration were assessed by means of light microscope. In the group investigated at day 3, there was statistically significant high tracheal revascularization in group III(p<0.05), but no difference at 7 and 14 days. And epithelial regenerations at day 3 were better in group III(p<0.05), and at day 7 in group II and III. But there was no difference at day 14. We concluded that b-FGF can enhance the revascularization and epithelial regeneration of the tracheal autograft especially in early phase.