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Screening and identification of H-2 d-restricted T cell epitopes in fusion and attachment glycoproteins of Nipah virus by ELISPOT / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology ; (12): 287-292, 2022.
Article in Chinese | WPRIM | ID: wpr-934045
ABSTRACT

Objective:

To screen and identify H-2 d-restricted T cell epitopes in fusion (F) and attachment (G) glycoproteins of Nipah virus (NiV) in mice.

Methods:

The complete peptides (single peptide contains 15 amino acids, and 10 amino acids were repeated in the front and back peptides) derived from F and G antigens were mixed into peptide libraries. BALB/c mice were immunized with DNA vaccines expressing NiV F and G proteins alone and in combination. The full sequence peptide libraries of F and G antigens were mixed into peptide pools by matrix design, and spleen cells of immunized mice were collected and analyzed by IFN-γ ELISPOT assay to detect the dominant H-2 d-restricted epitope peptides.

Results:

Twelve dominant H-2 d-restricted peptides were screened from the F protein-specific peptide library and the 56th peptide produced the strongest reaction. Four dominant peptides were screened from the G protein-specific peptide library and the 72nd peptide produced the strongest reaction.

Conclusions:

In this study, 12 F antigen-specific and 4 G antigen-specific H-2 d restricted dominant T cell epitopes of NiV were screened and identified by IFN-γ ELISPOT, which could provide reference for immunological analysis of NiV and vaccine research.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Screening study Language: Chinese Journal: Chinese Journal of Microbiology and Immunology Year: 2022 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Screening study Language: Chinese Journal: Chinese Journal of Microbiology and Immunology Year: 2022 Type: Article