Analysis of Gene Expression in Human Dermal Fibroblasts Treated with Senescence-Modulating COX Inhibitors
Genomics & Informatics
;
: 56-64, 2017.
Article
in English
| WPRIM
| ID: wpr-93440
ABSTRACT
We have previously reported that NS-398, a cyclooxygenase-2 (COX-2)–selective inhibitor, inhibited replicative cellular senescence in human dermal fibroblasts and skin aging in hairless mice. In contrast, celecoxib, another COX-2–selective inhibitor, and aspirin, a non-selective COX inhibitor, accelerated the senescence and aging. To figure out causal factors for the senescence-modulating effect of the inhibitors, we here performed cDNA microarray experiment and subsequent Gene Set Enrichment Analysis. The data showed that several senescence-related gene sets were regulated by the inhibitor treatment. NS-398 up-regulated gene sets involved in the tumor necrosis factor β receptor pathway and the fructose and mannose metabolism, whereas it down-regulated a gene set involved in protein secretion. Celecoxib up-regulated gene sets involved in G2M checkpoint and E2F targets. Aspirin up-regulated the gene set involved in protein secretion, and down-regulated gene sets involved in RNA transcription. These results suggest that COX inhibitors modulate cellular senescence by different mechanisms and will provide useful information to understand senescence-modulating mechanisms of COX inhibitors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aging
/
RNA
/
Gene Expression
/
Skin Aging
/
Aspirin
/
Genes, vif
/
Tumor Necrosis Factor-alpha
/
Cellular Senescence
/
Oligonucleotide Array Sequence Analysis
/
Cyclooxygenase 2
Limits:
Animals
/
Humans
Language:
English
Journal:
Genomics & Informatics
Year:
2017
Type:
Article
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