Effects of Cluster Scalp Acupuncture Combined with Enriched Environment Stimulationon Proliferationand Migration of Endogenous Neural Stem Cells in Neonatal Rats with Hypoxic-ischemic Brain Damage / 中国康复理论与实践

ABSTRACT

@#ObjectiveToexploretheeffectofclusterscalpacupuncturecombinedwithenrichedenvironmentalstimulationonprolifera-tionandmigrationofendogenousneuralstemcells(NSCs)inneonatalratswithhypoxic-ischemicbraindamage(HIBD).MethodsHIBD modelwasestablishedwithRicemethod.120ratswererandomlydividedintoshamoperationgroup(A),modelgroup(B),clusterscalpacu-puncturegroup(C),enrichedenvironmentalstimulationgroup(D)andclusterscalpacupuncturecombinedwithenrichedenvironmental stimulationgroup(E)with24ratsineachgroup,eachgroupwasdividedinto3days,7daysand14dayssubgroups.Immunohistochemical methodwasusedtoobservethepositivecellsofBromodeoxyuridine(BrdU)andnestin.ResultsComparedwithGroupB,thepositivecells numberofBrdUandnestininsubventricularzone(SVZ)andcerebralcortexincreasedinGroupsCandDatallthetimepoints(P<0.01), andthenumberwasmoreinGroupEthaninGroupsCandD(P<0.05).ConclusionClusterscalpacupuncturecombinedwithenricheden-vironmentalstimulationcouldpromotetheproliferationandmigrationofendogenousNSCsinSVZandcerebralcortexinneonatalratswith HIBD.