The in vivo imaging of zebrafish for chemical vascular toxicity assessment / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 33-37, 2022.
Article
in Chinese
| WPRIM
| ID: wpr-935247
ABSTRACT
Objective:
To evaluate the vascular toxicity of chemicals by a real-time observation approach using the transgenic zebrafish.Methods:
The spatiotemporal vascular alterations of transgenic zebrafish after chemical exposure were assessed by laser confocal microscopy and high-content screening analysis, respectively.Results:
The method using Laser Confocal Microscopy (LCM) is easier to operate and yields high-resolution images, while it is lower throughput and inefficient. In contrast, high-content analysis (HCA) analysis obtains high-quality data of vascular toxicity manifesting whole blood vasculature, whereas it requires delicate operation procedures and advanced experimental conditions.Conclusion:
Two kinds of zebrafish imaging methods each have advantages and disadvantages. LCM is suitable for the evaluation of a small number of chemicals. HCA, a cutting-edge technology, has great potential for chemical safety assessment allowing high throughput vascular toxicity tests of a good number of chemicals at a time.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Zebrafish
/
Animals, Genetically Modified
/
Cardiovascular System
/
Toxicity Tests
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2022
Type:
Article
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