Mechanism of Astragaloside Ⅳ in Treating Diabetic Retinopathy Based on Network Pharmacology and Molecular Docking / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
;
(24): 209-216, 2022.
Article
in Chinese
| WPRIM
| ID: wpr-940678
ABSTRACT
ObjectiveTo reveal the pharmacological mechanisms of astragaloside Ⅳ(AS-Ⅳ)in treating diabetic retinopathy based on network pharmacology and molecular docking and to provide reference for new drug development and mechanism research. MethodPotential targets of AS-Ⅳ were obtained from SwissTargetPrediction and Targetnet. The targets of diabetic retinopathy were screened using GeneCards,Online Mendelian Inheritance in Man(OMIM) and Therapeutic Target Database. The targets of AS-Ⅳ and diabetic retinopathy were intersected by Venny 2.1.0. STRING platform and Cytoscape 3.7.2 were used to construct protein-protein interaction(PPI) network and screen core targets, respectively. Then,Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. Furthermore,the binding affinity of AS-Ⅳ to key target receptors was assessed by molecular docking with Autodock Vina, and the key target signaling transduction pathway was ResultA total of 56 intersected targets of AS-Ⅳ and diabetic retinopathy were found,and the top five key targets were obtained through PPI network analysis:protein kinase B(Akt)1,vascular endothelial growth factor A(VEGFA),epidermal growth factor receptor(EGFR),Src and signal transducer and activator of transcription 3(STAT3). Molecular docking verified the strong binding affinity of AS-Ⅳ to the five key target receptors. In addition,in vitro tests have been confirmed that AS-Ⅳ attenuated high glucose-induced injury in human retinal pigment epithelial cell line ARPE-19 by regulating Akt/Nrf2/HO-1 and Akt/glycogen synthase kinase-3β(GSK-3β)signaling pathways. ConclusionThere was a significant overlap in the targets of AS-Ⅳ and diabetic retinopathy. The key targets and pathways may reveal the main pharmacological mechanism of AS-Ⅳ in the treatment of diabetic retinopathy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Experimental Traditional Medical Formulae
Year:
2022
Type:
Article
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