Effects of Achyranthis bidentatae -Cynanchum otophyllum on oxidative stress of cerebral tissue in Parkinson ’s disease mice with syndrome of hyperactivity of liver -Yang / 中国药房

ABSTRACT

OBJECTIVE To explore the effects of Achyranthis b identatae-Cynanchum otophyllum as core couplet medicinals of Zhengan xifeng decoction on oxidative stress of cerebral tissue in Parkinson ’s disease （PD）mice with syndrome of hyperactivity of liver-Yang. METHODS C57BL/6 mice were randomly divided into normal control group ，model group ，Zhengan xifeng decoction group，A. bidntatae group，C. otophyllum group and couplet medicinals of A. bidentatae-C. otophyllum group，with 10 mice in each group. PD model of hyperactivity of liver -Yang was established by intragastric administration of Aconitum carmichaelii decoction（4 g/kg） and intraperitoneal injection of 1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine （25 mg/kg）. The behavioral changes and ultrastructure of substantia nigra neurons in mice were observed . The expressions of tyrosine hydroxylase （TH）positive neurons in substantia nigra were detected . The expressions of total antioxidant capacity （T-AOC），superoxide dismutase （SOD） and malondialdehyde（MDA）in substantia nigra as well as mRNA and protein expressions of nuclear factor -erythroid 2-related factor 2 （Nrf2），thioredoxin reductase 1（Trxr1），thioredoxin interacting protein （Txnip）were also determined . RESULTS Compared with model group ，PD behavior and ultrastructure of substantia nigra neurons were all improved in administration groups . The expressions of TH positive neurons ，T-AOC（except for C.otophyllum group）and SOD （except for C.otophyllum group），mRNA relative expression and protein expression levels of Nrf 2 and Trxr 1 were all increased significantly ；the expression of MDA （except for C.otophyllum group）and mRNA relative expression and protein expression levels of Txnip were all decreased significantly （P＜0.05）. Intervention effect of couplet medicinals of A. bidentatae-C. otophyllum group was better than that of A.bidntatae group and C. otophyllum group（P＜ 0.05），and the effect was similar to that of decoction group （P＞0.05）. CONCLUSIONS The couplet medicinals of A. bidentatae-C. otophyllum can inhibit the level of oxidative stress in the cerebral tissue of PD mice with hyperactivity of liver -Yang by targeting Nrf 2，and play a protective role on the brain neurons . Its effect is better than that of A.bidntatae and C.otophyllum，and it plays the same role as that of the formula in tonifying the kidney ，softening the liver and suppressing the Yang .