Effects of Polyamines on TNFalpha- or Tamoxifen-induced Apoptosis in Human Breast Cancer Cells / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 385-391, 2001.
Article
in Korean
| WPRIM
| ID: wpr-94698
ABSTRACT
PURPOSE:
To investigate the effects of polyamines on tumor necrosis factor alpha (TNFalpha)-or tamoxifen (TAM)-induced apoptosis in estrogen receptor (ER)-positive MCF- 7 and ER-negative MDA-MB-231 human breast cancer cells. MATERIALS ANDMETHODS:
Cell viability was assessed by using MTT assay. Reactive oxygen species (ROS) generation was measured using 2', 7'-dichlorofluorescin diacetste (DCFDA) by fluorescence plate reader. DNA fragmentation was assessed by 1.5% agarose gel electrophoresis.RESULTS:
TNFalpah and TAM showed significant dose- and time- dependent inhibitory effects on the growth of MCF-7 human cells. However, the growth of MDA-MB-231 cells were not inhibited by TNFalpha or TAM treatment. The generation of ROS was increased in dose-and time-dependent manner by TNFalpha treatment in MCF-7 cells. Polyamines, especially spermine suppressed TNFalpha-induced ROS generation in MCF-7 cells. Antioxidant effects of polyamines were also demonstrated by DNA fragmentation, cell morphology as well as ROS generation assay. Polyamines also blocked TAM-induced cell death in MCF-7 cell. However, MDA-MB-231 cells showed resistance to the cytotoxic effects of TNFalpha or TAM.CONCLUSION:
These results suggest that polyamines may prevent TNFalpha or TAM-induced apoptosis in MCF-7 human breast cancer cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polyamines
/
Tamoxifen
/
Breast
/
Breast Neoplasms
/
Spermine
/
Cell Survival
/
Tumor Necrosis Factor-alpha
/
Cell Death
/
Reactive Oxygen Species
/
Apoptosis
Limits:
Humans
Language:
Korean
Journal:
Cancer Research and Treatment
Year:
2001
Type:
Article
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