Quantitative Analysis Of Random Chromosomal Aberrations In PHA-Stimulated Blood and Bone Marrow / 대한임상병리학회지
Korean Journal of Clinical Pathology
;
: 113-2000.
Article
in Korean
| WPRIM
| ID: wpr-94793
ABSTRACT
BACKGROUND:
Chromosomal aberration observed only in a few metaphases may cause the cytogeneticists to have difficulties in making a decision whether it is due to in vivo mosaicism/multiple clones or due to in vitro artifact. This is especially important when the chromosome of concern has been associated with a classical chromosome syndrome, malignancy or its evolution. Therefore, we aimed to establish a range for random chromosomal aberrations among cells from PHA-stimulated blood(PB) and bone marrow(BM) cultures.METHODS:
Among the cells from 449 PB and 472 BM specimens referred for chromosome studies from 1997 to 1998, we analyzed the frequency of random aneuploidy, structural abnormalities, and breaks/gaps.RESULTS:
The number of cells analyzed was 5,904/4,488(1997/1998) in PB and 4,211/4,124(1997/1998) in BM. The frequency of metaphases with random chromosomal aberrations of BM(32.10%) was much higher than that of PB(5.90%). The most frequent aberration was chromsome loss. Autosome losses were inversely correlated with autosome size(correlation coefficient = -0.83 and -0.72, p<0.01), smaller chromosomes being lost more frequently while autosome breaks/gaps were correlated with autosome size(correlation coefficient = 0.69 and 0.85, p<0.01), in PB and BM. Comparing the data from 1998 to the data from 1997, the frequency of chromosome losses(<0.5% in PB, <2.25% in BM), gains(<0.1% in PB and BM), breaks/gaps(<0.1% in PB, <0.25% in BM), and structural aberrations(
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Quality Control
/
Bone Marrow
/
Chromosome Aberrations
/
Chromosomes, Human, 4-5
/
Clone Cells
/
Artifacts
/
Aneuploidy
/
Metaphase
Type of study:
Controlled clinical trial
Language:
Korean
Journal:
Korean Journal of Clinical Pathology
Year:
2000
Type:
Article
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