Effects of black chokeberry extracts on metastasis and cell-cycle arrest in SK-Hep1 human liver cancer cell line

ABSTRACT

Objective: To determine the anti-cancer properties of black chokeberry extract on the SK- Hep1 human liver cancer cell line. Methods: MTT cell proliferation assay, wound migration, invasion, zymography and cell cycle were determined after black chokeberry fruit extract treatment. We also measured MMP-2/-9 and MT-1 MMP expression with protein and gene expression levels. Results: We detected four anthocyanins and three phenolic compounds in the black chokeberry by HPLC analysis. Cancer cell growth was inhibited in proportion to the concentration of black chokeberry extracts. In the adhesion test, 100 and 200 μg/mL of black chokeberry extracts decreased the adhesion rate of cancer cells to 87.6% and 75.3%, respectively, when the control group was 100.0%. The 200 μg/mL of black chokeberry extract reduced the MMP-2 and MMP-9 expressions up to 96.8%and 11.3%, respectively. Conclusions: Based on our results, in the SK-Hep1 liver cancer cells, the black chokeberry extract inhibits cancer cell proliferation, adhesion, and migration, ultimately inhibiting cancer metastasis.