Neutrophil extracellular traps in the intestinal mucosa of Eimeria-infected animals
Asian Pacific Journal of Tropical Biomedicine
;
(12): 301-307, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-950781
ABSTRACT
Objective:
To investigate the presence of neutrophil extracellular traps (NETs) in vivo by analysing intestinal sections from experimentally Eimeria bovis- and naturally Eimeria arloingi-infected animals.Methods:
Intestinal samples of Eimeria arloingi- and Eimeria bovis-infected animals were analysed by using immunohistochemical and fluorescence approach by using monoclonal antibodies.Results:
Classical NET components were confirmed by co-localization of extracellular DNA being decorated with neutrophil elastase and histones in Eimeria-infected tissue samples. Here, extrusion of NETs was exclusively detected in intestinal polymorphonuclear neutrophils infiltrating Eimeria-infected sites. In vivo NETs were either found in close proximity or in direct contact to different Eimeria stages suggesting a stage-independent process. NETs were also found within the gut lumen driven by polymorphonuclear neutrophils that were contacting released oocysts.Conclusions:
We postulate that NETs might play an important role in innate defence reactions in coccidiosis therefore significantly altering the outcome of infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Asian Pacific Journal of Tropical Biomedicine
Year:
2016
Type:
Article
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