In vitro antihistamine-releasing activity of a peptide derived from wasp venom of Vespa orientalis
Asian Pacific Journal of Tropical Biomedicine
;
(12): 259-264, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-950836
ABSTRACT
Objective:
To investigate the antihistamine-releasing effect of a peptide isolated from wasp venom of Vespa orientalis.Methods:
This peptide was separated from crude venom by chromatography methods and mass spectrometry. Then various concentrations (2, 4, 8, 16, 32, 64, 128 and 256 μmol/L) of the peptide were incubated with mast cells and lactate dehydrogenase assay was performed.Results:
No significant effect was observed in lactate dehydrogenase absorbance under 128 μmol/L concentration. This implied that the peptide did not cause cell death in mast cells and consequently, histamine release did not happen. Moreover, the results showed the IC
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Asian Pacific Journal of Tropical Biomedicine
Year:
2016
Type:
Article
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