Prevalence of shiga toxins (stx1, stx2), eaeA and hly genes of Escherichia coli O157: H7 strains among children with acute gastroenteritis in southern of Iran

ABSTRACT

Objective: To survey the prevalence severe diarrhea arising from these bacteria in children under 5 years old in Marvdasht. Methods: In this study faecal sample from 615 children aged 1, stx2, eaeA, hly has been analyzed. Results: E. coli O157H7 was detected in 7 (1.14%) stool specimens. A significant difference was seen between detection rate of isolated bacteria from age groups 18-23 months and other age groups (P=0.004). Out of considered virulence genes, only 1 of the isolated strains (0.16%) the stx1 and eaeA genes were seen and also all isolated bacteria had resistance to penicillin, ampicillin and erythromycin antibiotics. Conclusions: We found that children < 2 years of age were at highest risk of infection with E. coli O157H7. Regarding severity of E. coli O157H7 pathogenesis, low infectious dose and lack of routine assay for detection of these bacteria in clinical laboratory, further and completed studies on diagnosis and genotyping of this E. coli O157H7 strain has been recommended.