Immune stimulatory activity of BRP-4, an acidic polysaccharide from an edible plant, Basella rubra L
Asian Pacific Journal of Tropical Medicine
; (12): 849-853, 2014.
Article
in Zh
| WPRIM
| ID: wpr-951787
Responsible library:
WPRO
ABSTRACT
Objective: To evaluated the immunomodulatory effect of BRP-4, an acidic polysaccharide from Basella rubra (. B. rubra) L on the macrophage activity. Methods: Phagocytic activity was determined by the ingestion of Latex Beads-Rabbit IgG-FITC using the fluorescent microscopy and flow cytometry analysis and nitric oxide production was measured using Griess reaction assay. Results: An enhanced production of NO was observed at 10 and 100 μg/mL of BRP-4. The phagocytic activity of macrophage was enhanced in BRP-4 treated RAW264.7 cells. BRP-4 combined with concanavalin A (Con A) provided obvious promotion and strengthening of the proliferation of the splenocytes. Conclusions: BRP-4, polysaccharide isolated from B. rubra, is suggested to activate macrophage function and stimulate splenocyte proliferation. The strong immunomodulatory activity of BRP-4 confirmed its good potential as an immunotherapeutic adjuvant.
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Index:
WPRIM
Language:
Zh
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2014
Type:
Article