Specific primers design based on the superoxide dismutase b gene for Trypanosoma cruzi as a screening tool: Validation method using strains from Colombia classified according to their discrete typing unit
Asian Pacific Journal of Tropical Medicine
;
(12): 854-859, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-951788
ABSTRACT
Objective:
To classify 21 new isolates of Trypanosoma cruzi (. T. cruzi) according to the Discrete Typing Unit (DTU) which they belong to, as well as tune up a new pair of primers designed to detect the parasite in biological samples.Methods:
Strains were isolated, DNA extracted, and classified by using three Polymerase Chain Reactions (PCR). Subsequently this DNA was used along with other isolates of various biological samples, for a new PCR using primers designed. Finally, the amplified fragments were sequenced.Results:
It was observed the predominance of DTU I in Colombia, as well as the specificity of our primers for detection of T. cruzi, while no band was obtained when other species were used.Conclusions:
This work reveals the genetic variability of 21 new isolates of T. cruzi in Colombia.Our primers confirmed their specificity for detecting the presence of T. cruzi.
Full text:
Available
Index:
WPRIM (Western Pacific)
Country/Region as subject:
South America
/
Colombia
Language:
Chinese
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2014
Type:
Article
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