The role of mapk and pkc-delta in phosphatidic acid-mediated intercellular adhesion molecule-1 expression
Journal of the Korean Association of Oral and Maxillofacial Surgeons
;
: 445-454, 2007.
Article
in Korean
| WPRIM
| ID: wpr-95183
ABSTRACT
BACKGROUND:
Phosphatidic acid (PA), an important second messenger, is involved in inflammation. Notably, cell-cell interactions via adhesion molecules play a central role in inflammation. This thesis show that PA induces expression of intercellular adhesion molecule-1 (ICAM-1) on macrophages and describe the signaling pathways. MATERIALS ANDMETHODS:
Macrophages were cultured in the presence of 10% FBS and assayed cell to cell adhesion using HUVEC. For the gene and protein analysis, RT-PCR, Western blot and flow cytometry were performed. In addition, overexpressed cell lines for dominant negative PKC-delta mutant established and tested their effect on the promoter activity and expression of ICAM-1 protein by PA.RESULTS:
PA-activated macrophages significantly increased adhering to human umbilical vein endothelial cell and this adhesion was mediated by ICAM-1. Pretreatment with rottlerin (PKC-delta inhibitor) or expression of a dominant negative PKC-delta mutant, but not Go6976 (classical PKC-alpha inhibitor) and myristoylated PKC-zeta inhibitor, attenuated PA-induced ICAM-1 expression. The p38 mitogen-activated protein kinase (MAPK) inhibitor blocked PA-induced ICAM-1 expression in contrast, ERK upstream inhibitor didn't block ICAM-1.CONCLUSION:
These data suggest that PA-induced ICAM-1 expression and cell-cell adhesion in macrophages requires PKC-delta activation and that PKC-delta activation is triggers to sequential activation of p38 MAPK.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphatidic Acids
/
Protein Kinases
/
Umbilical Veins
/
Protein Kinase C
/
Second Messenger Systems
/
Cell Adhesion
/
Cell Line
/
Blotting, Western
/
Intercellular Adhesion Molecule-1
/
Endothelial Cells
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Association of Oral and Maxillofacial Surgeons
Year:
2007
Type:
Article
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