Change of Intracellular Calcium in Myocardial Cell of Obesity Prone Rats / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
;
(12): 426-428, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-953882
ABSTRACT
@#Objective To study the intracellular calcium ([Ca2+ ]i) in myocardial cell of obesity rats induced by high-fat diet. Methods Male Sprague-Dawley (SD) rats were divided into obesity resistant (OR, n=15), normal (Nor, n=15) and obesity prone (OP, n=15) group after fed with high-fat diet for 10 weeks. Their body fat and serum lipids were measured. Myocardial cells were isolated with Langendorff perfusion and [Ca2+]i was measured with calcium indicator Fluo-3/AM and laser scanning confocal microscope after KCl depolarization and caffeine- induced. Results Compared with those in Nor and OR rats, the epididymal fat, perirenal fat, omental fat and body fat increased in OP rats (P<0.05), as well as the the level of total cholesterol, triglyceride and low density lipoprotein (P<0.05); the vary of [Ca2+]i elevation and restoration were lower (P<0.05). Conclusion The vary of [Ca2+ ]i elevation decreases in OP rats after KCl depolarization and caffeine-induced, that may associated with arrhythmia in obesity rats.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Rehabilitation Theory and Practice
Year:
2011
Type:
Article
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