Value of LncRNA PRNCR1 in peripheral blood mononuclear cells as a diagnostic marker for osteoporosis and its relationship with inflammatory factors in patients / 中华内分泌外科杂志

ABSTRACT

Objective:To investigate the value of LncRNA PRNCR1 in peripheral blood mononuclear cells (PBMC) as a diagnostic marker for osteoporosis and its relationship with inflammatory factors in patients.Methods:The expression of LncRNA PRNCR1 in PBMCs of healthy controls and OP patients was detected by qRT-PCR and its diagnostic value in OP was explored. Enzyme linked immunosorbent assay (ELISA) was used to detect the concentrations of IL-6 and TNF-α in PBMC, and the correlation between LncRNA PRNCR1 and IL-6 and TNF-α concentrations was analyzed. LncRNA PRNCR1 was overexpressed or knocked down and its effect on inflammatory factor concentration was verified. The relationship between the expression of LncRNA PRNCR1 and the treatment days, recurrence rate of patients was analyzed, and the possible mechanism of LncRNA PRNCR1 affecting the progression of OP was explored through bioinformatics.Results:Compared with healthy control group, the expression of LncRNA PRNCR1 in PBMCs of OP group was significantly enhanced and could be used as a marker for the diagnosis of OP ( t=8.42, P<0.001) (AUC=0.83, P<0.001) . In addition, the concentrations of IL-6 and TNF-α in PBMCs of OP group were significantly enhanced and positively correlated with the expression of LncRNA PRNCR1 ( r=0.25, P=0.016) ( r=0.21, P=0.037) . Overexpression of LncRNA PRNCR1 promoted the expression of inflammatory factors while knockdown of LncRNA PRNCR1 inhibited the expression of inflammatory factors. Patients with high expression of LncRNA PRNCR1 remained on treatment longer than those with low expression. The results of bioinformatics analysis showed that LncRNA PRNCR1 may affect OP progression by affecting stem cell differentiation pathway and estrogen signaling pathway. Conclusions:The expression of LncRNA PRNCR1 in PBMC of patients with OP is significantly enhanced. LncRNA PRNCR1 can be used as an important marker for the diagnosis of OP and affects the expression of inflammatory factors.