Correlation between caspase recruitment domain protein 9 gene polymorphism and susceptibility to sepsis related liver injury / 中国综合临床

ABSTRACT

Objective:To investigate the relationship between caspase recruitment domain protein 9 (CARD9) gene polymorphism and the risk of sepsis related liver injury.Methods:A case-control study was conducted to recruit 122 patients with sepsis in intensive care unit (ICU). The septic patients with liver injury were divided into the liver injury group ( n=66), and the patients without liver injury were set as the control group ( n=60). TaqMan genotyping technique was performed to detected the six single nucleotide polymorphisms of CARD9 gene in peripheral blood, and the baseline data of the two groups were observed. The differences of genotype distribution and allele frequency between the two groups were compared, and the odds ratios ( OR) were calculated. Kolmogorov-Smirnov test was used for normal distribution test, the measurement data that do not conform to the normal distribution are expressed in M ( Q1, Q3), and the Mann Whitney U test was used for the comparison between the two groups. The comparison of counting data between groups was adopted χ 2 inspection and Fisher exact probability method. χ 2 test is used to determine whether the genotype distribution conforms to hardy Weinberg equilibrium test. Results:Compared with the control group, the CC genotype distribution of rs10781500 locus of CARD9 gene in liver injury group was significantly increased (19 vs. 36, χ 2=6.87, P=0.033), and sepsis patients with C allele are more likely to induce liver injury ( OR=1.375, 95% CI 1.024-1.846, P=0.023). Patients with CC genotype have a higher risk of liver injury ( OR=2.696, 95% CI 1.238-5.869, P=0.012). In the liver injury group, the alanine aminotransferase and SOFA score in CC genotype at re10781500 locus were significantly higher than those in non-CC genotype (310 (213, 648) U/L vs. 187 (114, 304) U/L, Z=3.32, P=0.001 and 9 (6, 14) points vs. 7 (5, 9) points, Z=2.49, P=0.013). Conclusion:The CC genotype at rs10781500 locus of CARD9 gene was associated with sepsis-induced liver injury, and the C allele may be a susceptibility gene.