Wenbu Gushen Recipe alleviates renal fibrosis and pyroptosis by down-regulating CTRP6 in rats with diabetic nephropathy / 中华内分泌代谢杂志

ABSTRACT

Objective:To explore the effect and mechanism of Wenbu Gushen Recipe on renal fibrosis and pyroptosis in diabetic nephropathy (DN) rats.Methods:Sixty SD rats were randomly divided into control group, model group, low, medium and high dose groups of Wenbu Gushen Decoction, irbesartan group with 10 rats each. Except for control group, other groups were given intraperitoneal injection of streptozotocin (60 mg/kg) to construct model the DN. The low-dose, middle-dose, and high-dose groups were given different doses of Wenbu Gushen(0.92, 1.84, and 3.68 g·kg -1·d -1) respectively, irbesarta group was gavaged with 13.5 mg·kg -1·d -1 of . The rats were given the same amount of normal saline in the control group and the model group. After 12 weeks of treatment, 24 hour urine protein, urea nitrogen, creatinine and blood glucose of each group of rats were measured; the pathological changes of rat kidneys were observed; The expression of collagen Ⅲ, transforming growth factor(TGF)-β1, pro-casapse-1, cleaved-caspase-1, GSDMD, GSDMD-N, interleukin(IL)-1β, IL-18, and C1q/tumor necrosis factor-related protein 6 (CTRP6) were detected in kidney tissues; ELISA to detect IL-1β, IL-18 concentration in serum. Results:Compared with control group, the 24 hour urine protein, urea nitrogen, creatinine and blood glucose were significantly increased in the model group; The kidney tissue showed obvious pathological changes, and the expression of TGF-β1, collagen Ⅲ, cleaved-caspase-1, GSDMD-N, IL-1β, IL-18 and CTRP6 were increased in renal tissues, accompanied with IL-1β and IL-18 concentration were increased in serum. Compared with the model group, Wenbu Gushen Decoction inhibited the above indicators and improve kidney injury.Conclusion:Wenbu Gushen Recipe has a protective effect on DN renal injury, and it may inhibit renal fibrosis and pyrpoptosis via down-regulation of CTRP6.