Generation of Renal Cell Carcinoma-specific CD4+ /CD8+ T Cells Restricted by an HLA-39 from a RCC Patient Vaccinated with GM-CSF Gene-Transduced Tumor Cells
Immune Network
;
: 96-102, 2003.
Article
in English
| WPRIM
| ID: wpr-9585
ABSTRACT
BACKGROUND:
Granulocyte-macrophage colony-stimulating factor (GM-CSF) gene- transduced tumor cell vaccines induce very potent systemic anti-tumor immunity in preclinical and clinical models. Our previous phase I clinical trial in patients with metastatic renal cell carcinoma (RCC) has demonstrated both immune cell infiltration at vaccine sites and T cell-mediated delayed-type hypersensitivity (DTH) response to whole tumor cell vaccines.METHODS:
To investigate the immune responses to autologous genetically- modified tumor cell vaccines, tumor-specific CD8+ T cell lines were generated from peripheral blood lymphocytes (PBL) of a RCC patient 1.24 by repeated in vitro stimulation with either B7.1-transduced autologous RCC tumor cells or B7.1-transduced autologous tumor cells treated with interferon gamma (IFNgamma), and cloned by limiting dilution.RESULTS:
Among several RCC-specific cytotoxic T lymphocytes (CTLs), a CD4+ /CD8+ double positive T cell clone (17/A2) appeared to recognize IFNgamma-treated autologous RCC restricted by HLA-B39. The 17/A2 also recognized other HLA-B39 positive RCC tumor cells after IFNgamma treatment.CONCLUSION:
These results demonstrate that autologous RCC vaccination successfully generates the tumor-specific CTL 17/A2, and suggest that the presentation and recognition of the tumor antigen by the 17/A2 might be upregulated by IFNgamma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Vaccines
/
Lymphocytes
/
Carcinoma, Renal Cell
/
T-Lymphocytes
/
T-Lymphocytes, Cytotoxic
/
Cell Line
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Interferons
/
Vaccination
/
Clone Cells
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Immune Network
Year:
2003
Type:
Article
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