Identification of differentially expressed cDNAs in Acanthamoeba culbertsoni after mouse brain passage
The Korean Journal of Parasitology
;
: 15-20, 2006.
Article
in English
| WPRIM
| ID: wpr-96038
ABSTRACT
Free-living amoebae of the genus Acanthamoeba are causative agents of granulomatous amebic encephalitis and amebic keratitis. Because the virulence of Acanthamoeba culbertsoni cultured in the laboratory is restored by consecutive brain passages, we examined the genes induced in mouse brain-passaged A. culbertsoni by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). Enhanced A. culbertsoni virulence was observed during the second mouse brain passage, i.e., infected mouse mortality increased from 5% to 70%. Ten cDNAs induced during mouse brain passage were identified by DDRT-PCR and this was confirmed by northern blot analysis. BlastX searches of these cDNAs indicated the upregulations of genes encoding predictive NADH-dehydrogenase, proteasomal ATPase, and GDP-mannose pyrophosphorylase B, which have previously been reported to be associated with A. culbertsoni virulence factors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virulence
/
Brain
/
Acanthamoeba
/
Molecular Sequence Data
/
Serial Passage
/
Up-Regulation
/
Gene Expression Regulation
/
Blotting, Northern
/
DNA, Protozoan
/
Cloning, Molecular
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2006
Type:
Article
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