Effect of mannitol on testicular ischemia-reperfusion injury: An experimental an

ABSTRACT

Objective: To determine the effect of mannitol in reducing testicular damage after ischemia-reperfusion (I-R) injury in rabbitsMaterials and Methods: three groups of male rabbits were included in the study 1. Baseline group (n=6), underwent bilateral orchiectomy to serve as baseline; 2. Control group (n=24), testicular torsion simulated for 4 hours; 3 Mannitol group (n=24), testicular torsion simulated for 4 hours and mannitol administered during the last 30 minutes of ischemia. The latter two (2) groups underwent bilateral orchiectomy after one week. Architecture and germinal epithelial cell thickness were determined by histological examination on each testis. Average thickness (in cell layers) of the mannitol group was compared with control group using the T-testResults: The mannitol group showed a significant increase in epithelial cell thickness compared with control group (4.8 +/- 0.09 versus 3.0 +/- 0.1 cell layers, p0.01)Conclusion: Mannitol therapy administered during testicular ischemia-reperfusion injury significantly reduced damage to the testis in this animal model. (Author)