Suppression of nitric oxide and interleukin-6 production by methanol extract of Sophorae Flos in macrophage cells / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
;
: 9-19, 2005.
Article
in Korean
| WPRIM
| ID: wpr-96282
ABSTRACT
Both nitric oxide (NO) and interleukin-6 (IL-6) have been thought to have a role in the pathogenesis of inflammatory periodontal disease as it does in other inflammatory diseases, and the inhibitors of NO and IL-6 production have been considered as potential anti-inflammatory agents. In this study, we evaluated methanol extract of Sophorae Flos for inhibition of NO and IL-6 production in Prevotella intermedia LPS-induced mouse macrophages RAW264.7 cells. Dried Sophorae Flos was sliced, and extracted with 100% methanol. LPS from P. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. NO production was assayed by measuring the accumulation of nitrite in culture supernatants and IL-6 was measured using mouse IL-6 ELISA kit. Western blot analysis of iNOS and analysis of reverse transcription (RT)-PCR products were carried out. The methanol extract of Sophorae Flos concentration-dependently reduced the production of NO and the expression of iNOS protein and mRNA in RAW264.7 cells treated with P. intermedia LPS. Sophorae Flos also suppressed IL-6 production and the expression of IL-6 mRNA in RAW264.7 cells stimulated by P. intermedia LPS. The inhibition of NO and IL-6 production by Sophorae Flos may be useful in the therapy of inflammatory diseases such as periodontitis. This hypothesis, however, remains to be tested.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Periodontal Diseases
/
Periodontitis
/
RNA, Messenger
/
Enzyme-Linked Immunosorbent Assay
/
Blotting, Western
/
Interleukin-6
/
Prevotella intermedia
/
Sophora
/
Reverse Transcription
/
Methanol
Limits:
Animals
Language:
Korean
Journal:
The Journal of the Korean Academy of Periodontology
Year:
2005
Type:
Article
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