Development of PCR Technology for Identification of the Restriction Fragment Length Polymorphism(RFLP) of the Immunoglobulin Allotypes in Periodontal Patients / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
;
: 349-354, 1999.
Article
in Korean
| WPRIM
| ID: wpr-96293
ABSTRACT
The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Restriction Fragment Length
/
DNA
/
Immunoglobulins
/
Lymphocytes
/
Polymerase Chain Reaction
/
Genetics, Population
/
Immunoglobulin Allotypes
Type of study:
Diagnostic study
Limits:
Humans
Language:
Korean
Journal:
The Journal of the Korean Academy of Periodontology
Year:
1999
Type:
Article
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