Heterogeneous Composition of Voltage-Dependent K+ Currents in Hepatic Stellate Cells
Yonsei Medical Journal
;
: 684-693, 2007.
Article
in English
| WPRIM
| ID: wpr-96526
ABSTRACT
PURPOSE:
Hepatic stellate cells (HSC) are a type of pericyte with varying characteristics according to their location. However, the electrophysiological properties of HSC are not completely understood. Therefore, this study investigated the difference in the voltage-dependent K(+) currents in HSC. MATERIALS ANDMETHODS:
The voltage-dependent K(+) currents in rat HSC were evaluated using the whole cell configuration of the patch-clamp technique.RESULTS:
Four different types of voltage-dependent K(+) currents in HSC were identified based on the outward and inward K(+) currents. Type D had the dominant delayed rectifier K(+) current, and type A had the dominant transient outward K(+) current. Type I had an inwardly rectifying K(+) current, whereas the non-type I did not. TEA (5mM) and 4-AP (2mM) suppressed the outward K(+) currents differentially in type D and A. Changing the holding potential from -80 to -40mV reduced the amplitude of the transient outward K(+) currents in type A. The inwardly rectifying K(+) currents either declined markedly or were sustained in type I during the hyperpolarizing step pulses from -120 to -150mV.CONCLUSION:
There are four different configurations of voltage-dependent K(+) currents expressed in cultured HSC. These results are expected to provide information that will help determine the properties of the K(+) currents in HSC as well as the different type HSC populations.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cells, Cultured
/
Ion Transport
/
Patch-Clamp Techniques
/
Hepatocytes
/
Potassium Channels, Voltage-Gated
/
Electric Conductivity
Limits:
Animals
Language:
English
Journal:
Yonsei Medical Journal
Year:
2007
Type:
Article
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