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Application of CRISPR/Cas systems in the nucleic acid detection of pathogens: a review / 中国血吸虫病防治杂志
Chinese Journal of Schistosomiasis Control ; (6): 98-103, 2023.
Article in Chinese | WPRIM | ID: wpr-965536
ABSTRACT
Rapid, sensitive and specific detection tools are critical for the prevention and control of infectious diseases. The in vitro nucleic acid amplification assays, including polymerase chain reaction and isothermal amplification technology, have been widely used for the detection of pathogens. Recently, nucleic acid detection-based on clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) have been developed, which are rapid, highly sensitive, highly specific, and portable. This review describes the classification and principle of CRISPR/Cas systems and their applications in pathogen detection, and discusses the prospects of CRISPR/Cas systems.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Schistosomiasis Control Year: 2023 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Schistosomiasis Control Year: 2023 Type: Article