Delayed allograft rejection by the suppression of class II transactivator
Experimental & Molecular Medicine
;
: 210-216, 2006.
Article
in English
| WPRIM
| ID: wpr-96570
ABSTRACT
We examined the effect of class II transactivator (CIITA) down-modulation on allograft rejection. To inhibit the function of CIITA, we constructed a series of CIITA mutants and found one exhibiting the dominant-negative effect on the regulation of major histocompatibility complex (MHC) class II expression. To test whether the CIITA dominant-negative mutant reduces immunogenecity, CIITA-transfected melanoma cells were injected into allogeneic host and assessed for immune evading activity against host immune cells. We demonstrated that the CIITA dominant-negative mutant allowed tumor nodules to develop earlier in the lung than control by this tumor challenge study. Furthermore, skin grafts deficient for CIITA also survived longer than wild-type in allogeneic hosts. Both the tumor challenge and skin graft studies suggest the inhibition of CIITA molecules in donor tissue would be beneficial to the control of allo-response.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transplantation, Homologous
/
Melanoma, Experimental
/
Mice, Transgenic
/
Nuclear Proteins
/
Transfection
/
Histocompatibility Antigens Class II
/
Transcriptional Activation
/
Trans-Activators
/
Interferon-gamma
/
Skin Transplantation
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2006
Type:
Article
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