Identification of Novel Genes with Proapoptotic Activity
Genomics & Informatics
;
: 77-79, 2006.
Article
in English
| WPRIM
| ID: wpr-96576
ABSTRACT
In order to identify novel proapoptotic genes, we screened approximately 1,000 hypothetical genes whose functions are completely unknown. After these genes were transiently expressed in HeLa cells, their nuclei images were captured using automated high-speed fluorescence microscope, through which the ratio of apoptotic nuclei was estimated. We selected genes that induce greater than 3-fold increase in apoptotic nuclei compared to that of the vector control. The candidate proapoptotic genes were sequenced and their effects on cell death were further confirmed by the additional assay, DNA fragmentation ELISA. Finally, we were able to identify 4 full-length hypo-thetical genes with proapoptotic activity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Enzyme-Linked Immunosorbent Assay
/
HeLa Cells
/
Cell Death
/
Apoptosis
/
DNA Fragmentation
/
Fluorescence
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Genomics & Informatics
Year:
2006
Type:
Article
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