ras Gene Mutations in Malignant Fibrous Histiocytoma
Korean Journal of Pathology
;
: 232-237, 2001.
Article
in Korean
| WPRIM
| ID: wpr-96590
ABSTRACT
BACKGROUND:
ras gene mutations have been described in various human malignancies, suggesting that their activation may play a role in oncogenesis. However, there are few reports concerning ras gene alterations in malignant fibrous histiocytomas. We therefore designed a study to determine the prevalence and type of mutations in the first exons of H-ras and K-ras genes in these tumors.METHODS:
Twenty-seven malignant fibrous histiocytomas were investigated by direct sequencing analysis with the automated DNA sequencing of polymerase chain reaction-amplified ras sequences.RESULTS:
Twenty-four mutations were found in 18 (67%) of the tumors GGC to GAC transition mutations at codon 13 of K-ras (coding for aspartic acid instead of glycine) in 18 of the samples and GGC to GTC transversions at codon 12 of H-ras (coding for valine instead of glycine) in six of the lesions.CONCLUSIONS:
Our data suggest an involvement of the ras gene mutation in conjunction with other yet unknown events in the tumorigenesis and/or progression of malignant fibrous histiocytomas. The K-ras gene activation predominated in these tumors by a mutation at codon 13. It is noteworthy that H-ras mutations were detected only in association with the lesions containing K-ras mutated genes, the significance of which remains to be determined.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Valine
/
Codon
/
Exons
/
Prevalence
/
Genes, ras
/
Sequence Analysis, DNA
/
Aspartic Acid
/
Histiocytoma, Malignant Fibrous
/
Carcinogenesis
Type of study:
Prevalence study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Pathology
Year:
2001
Type:
Article
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