Altered Cholecystokinin-induced Calcium Signal in Streptozotocin-induced Diabetic Rat Pancreatic Acini / 대한소화기학회지
The Korean Journal of Gastroenterology
;
: 519-526, 2003.
Article
in Korean
| WPRIM
| ID: wpr-96871
ABSTRACT
BACKGROUND/AIMS:
Pancreatic acini of streptozotocin (STZ)-induced diabetic rats release amylase less than normal acini on cholecystokinin (CCK) stimulation. Pancreatic enzyme secretion has been closely related to the intracellular calcium concentration ([Ca2+]i) of the acinar cell. In the present study, sequential changes of the intracellular calcium signal which probably underlie the altered enzyme secretion in response to CCK-8 were investigated using pancreatic acini from diabetic rats.METHODS:
Diabetic rats were prepared by single intravenous injection of STZ (70 mg/kg). Stimulating experiments with CCK-8 were performed 7 days later. Pancreatic acini were isolated by collagenase digestion. Amylase release and [Ca2+]i were measured by colorimethod and calcium imaging, respectively. The geometry of intracellular calcium signal was analyzed.RESULTS:
Normal acini exhibited concentration-dependent [Ca2+]i increase and regular oscillatory calcium signal on CCK-8 stimulation. Amylase release was also concentration-dependent. However, diabetic acini showed significantly less [Ca2+]i increase, prolonged time to peak [Ca2+]i, decreased calcium spikes number, and decreased amylase release compared with normal acini. The decreased [Ca2+]i in diabetic acini was restored significantly by insulin treatment.CONCLUSIONS:
Relatively decreased amylase release in diabetic pancreatic acini in response to CCK, appears to be associated with altered calcium signal due to insulin deficiency.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreas
/
Sincalide
/
Rats, Sprague-Dawley
/
Calcium Signaling
/
Diabetes Mellitus, Experimental
/
Amylases
Limits:
Animals
Language:
Korean
Journal:
The Korean Journal of Gastroenterology
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS