Expression of Hepatitis B Virus X Protein in Hepatocytes Suppresses CD8+ T Cell Activity
Immune Network
;
: 126-134, 2010.
Article
in English
| WPRIM
| ID: wpr-96923
ABSTRACT
BACKGROUND:
CD8+ T cells contribute to the clearance of Hepatitis B virus (HBV) infection and an insufficient CD8+ T cell response may be one of the major factors leading to chronic HBV infection. Since the HBx antigen of HBV can up-regulate cellular expression of several immunomodulatory molecules, we hypothesized that HBx expression in hepatocytes might affect CD8+ T cell activity.METHODS:
We analyzed the activation and apoptosis of CD8+ T cells co-cultured with primary hepatocytes rendered capable of expressing HBx by recombinant baculovirus infection.RESULTS:
Expression of HBx in hepatocytes induced low production of interferon-gamma and apoptosis of CD8+ T cells, with no effect on CD8 T cell proliferation. However, transcriptional levels of H-2K, ICAM-1 and PD-1 ligand did not correlate with HBx expression in hepatocytes.CONCLUSION:
Our results suggest that HBx may inhibit CD8+ T cell response by regulation of interferon-gamma production and apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Viral Proteins
/
T-Lymphocytes
/
Hepadnaviridae
/
Trans-Activators
/
Hepatitis B virus
/
Baculoviridae
/
Interferon-gamma
/
Apoptosis
/
Intercellular Adhesion Molecule-1
/
Hepatocytes
Language:
English
Journal:
Immune Network
Year:
2010
Type:
Article
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