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Search of Altered Gene Expression after Chronic Administration of Olanzapine in the Rat Frontal Cortex using cDNA Microarray / 대한정신약물학회지
Korean Journal of Psychopharmacology ; : 360-366, 2003.
Article in Korean | WPRIM | ID: wpr-97031
ABSTRACT

OBJECTIVE:

cDNA microarray is a convenient molecular technology that enables to search for gene expression in large scale. To explore the effect of antipsychotics on the gene expression in the brain, we applied cDNA microarray and searched for differentially expressed genes in the olanzapine-treated rat frontal cortex.

METHODS:

We administered olanzapine (4 mg/kg/day, IP) to S-D rats for 14days, and dissected the frontal cortex to examine. We analyzed altered gene expression from microarray, and screened up- or down-regulated genes. Their changes were confirmed by RT-PCR.

RESULTS:

Three down-regulated and one up-regulated genes were screened by triplicate cDNA microarray analysis. Among them, translocase of the inner mitochondrial membrane 23 (TIM23) was confirmed in RT-PCR. The expression of TIM23 mRNA was significantly increased in olanzapine-treated rat frontal cortex.

CONCLUSION:

This is the first report of up-regulated gene expression of TIM23 by antipsychotics in the rat brain. TIM23 is the essential component of mitochondrial biogenesis. From this result, we suggest that antipsychotic effect may be related to the improvement of mitochondrial dysfunction in the brain.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Schizophrenia / Antipsychotic Agents / Organelle Biogenesis / Brain / RNA, Messenger / Gene Expression / DNA, Complementary / Oligonucleotide Array Sequence Analysis / Mitochondrial Membranes Limits: Animals Language: Korean Journal: Korean Journal of Psychopharmacology Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Schizophrenia / Antipsychotic Agents / Organelle Biogenesis / Brain / RNA, Messenger / Gene Expression / DNA, Complementary / Oligonucleotide Array Sequence Analysis / Mitochondrial Membranes Limits: Animals Language: Korean Journal: Korean Journal of Psychopharmacology Year: 2003 Type: Article