Lipase inhibitory activity of Lagenaria siceraria fruit as a strategy to treat obesity

ABSTRACT

Objective To explore pancreatic lipase inhibitory activity under different extraction conditions in order to track the most potent extract. Methods The methanolic extract and its fractions in solvents of increasing polarity, ether, chloroform, ethyl acetate, n-butanol and water, were made through cold maceration. Extracts in ethanol, ethyl acetate, acetone and chloroform were similarly prepared. Aqueous extract was prepared through hot decoction method. A reported method was used to determine lipase inhibitory activity of extracts and fractions over wide ranges of concentrations. Results The extracts and fractions exhibited concentration dependent activity. The IC