Adherence and internalisation of Lactococcus lactis M4 towards human colorectal cancer cell line, Caco-2
Malaysian Journal of Microbiology
;
: 321-325, 2021.
Article
in English
| WPRIM
| ID: wpr-972796
ABSTRACT
Aims@#Lactococcus lactis is a non-colonizing, generally-regarded as safe (GRAS) lactic acid bacteria that has been frequently studied as a potential vector for bactofection. To mediate bactofection, a series of interaction between the bacteria and the host cell needs to occur. This study aims to investigate the in vitro bacterial-cell interaction between a locally-isolated L. lactis M4 strain with human colorectal cancer line, Caco-2.@*Methodology and results@#Bacterial interaction was evaluated via adherence and internalisation assays. A 2501 ratio of bacteria to cancer cell was selected as the optimum multiplicity of infection for all assays. After 2 h, L. lactis M4 was able to adhere to and internalise into Caco-2 cells at comparable rates to commercial strains L. lactis NZ9000 and MG1363. @*Conclusion, significance and impact of study@#Findings from this study showed that this strain has similar interaction properties with the commercial strains and would make a promising candidate for future bactofection studies and development of bacteria-mediated DNA vaccination against various diseases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Colorectal Neoplasms
/
Lactococcus lactis
/
Caco-2 Cells
Language:
English
Journal:
Malaysian Journal of Microbiology
Year:
2021
Type:
Article
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