Experimental Study of Efficient Transduction for Human Pulmonary Artery Smooth Muscle Cells with HIV-based Lentivirus Vectors / 中国康复理论与实践

ABSTRACT

@# Objective To infect human pulmonary artery smooth muscle cells with different HIV-based vectors, and introduce foreign genes into the cells with HIV-based lentivirus vectors.MethodsThe human pulmonary artery smooth muscle cells, U87.CD4.CCR5 and U87.CD4.CXCR4, were infected with ADA/Luc, HXB2/Luc, VSV-G/Luc and VSV-G/GFP.ResultsU87.CD4 with corresponding coreceptors could be infected by ADA/Luc and HXB2/Luc, however, the human pulmonary artery smooth muscle cells could not be infected by ADA/Luc and HXB2/Luc, even pseudotypes. VSV-G/Luc and VSV-G/GFP could be transfered into the human pulmonary artery smooth muscle cells. After 3 days post-infection, the relative proportion of positive cells of human pulmonary artery smooth muscle cells expressing VSV-G/GFP was 18%.ConclusionHIV-based lentivirus vectors can introduce foreign genes into the human pulmonary artery smooth muscle cells.